Chinese Journal OF Rice Science ›› 2017, Vol. 31 ›› Issue (4): 345-354.DOI: 10.16819/j.1001-7216.2017.6165 345
• Orginal Article • Previous Articles Next Articles
Chenxing ZHAO, Yewei YU, Yipeng XU, Xiaoping YU*()
Received:
2016-12-12
Revised:
2017-03-22
Online:
2017-07-25
Published:
2017-07-10
Contact:
Xiaoping YU
通讯作者:
俞晓平
基金资助:
Chenxing ZHAO, Yewei YU, Yipeng XU, Xiaoping YU. Gene Cloning, Polyclonal Antibody Preparation and Expression Localization of Two dynamin-1-like Genes from Nilaparvata lugens (Hemiptera: Delphacidae)[J]. Chinese Journal OF Rice Science, 2017, 31(4): 345-354.
赵晨星, 俞叶微, 许益鹏, 俞晓平. 褐飞虱两个dynamin-1-like基因的克隆、多克隆抗体制备及 表达定位[J]. 中国水稻科学, 2017, 31(4): 345-354.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2017.6165 345
引物名称 Primer name | 正向引物(5′-3′) Forward sequence(5′-3′) | 反向引物(5′-3′) Reverse sequence(5′-3′) |
---|---|---|
基因克隆 Gene clone | ||
NlDNM1L-1 | GGCTTTGTTGGTGCTTTCA | CGAACCGGAATAGCCTTCT |
NlDNM1L-2 原核表达 Prokaryotic expression NlDNM1L-1 NlDNM1L-2 定量PCR qPCR qNlDNM1L-1 qNlDNM1L-2 qNl18SrRNA | CTTTCTTGCGATTGTCACGC GGATCCATGGAAGCTCTTATACCAGTTACCA GAATTCATGGAAGCCTTGATACCAGTGATAA CGGCACCGATATTATACAGCTTC CAAAGCAGCGACAACGAATC GTAACCCGCTGAACCTCC | GGCCCAAAAGGAATCAACTA AAGCTTCTACCACAGATGTATTTCTCTGATCT AAGCTTCTACCACAGGTGAGTCTCGCGAACT AAGTTGAAGAACCAAGGGACACC CTCAGGCAGAAGATTGATGGG GTCCGAAGACCTCACTAAATCA |
Table 1 Primer sequences used for gene clone and prokaryotic expression.
引物名称 Primer name | 正向引物(5′-3′) Forward sequence(5′-3′) | 反向引物(5′-3′) Reverse sequence(5′-3′) |
---|---|---|
基因克隆 Gene clone | ||
NlDNM1L-1 | GGCTTTGTTGGTGCTTTCA | CGAACCGGAATAGCCTTCT |
NlDNM1L-2 原核表达 Prokaryotic expression NlDNM1L-1 NlDNM1L-2 定量PCR qPCR qNlDNM1L-1 qNlDNM1L-2 qNl18SrRNA | CTTTCTTGCGATTGTCACGC GGATCCATGGAAGCTCTTATACCAGTTACCA GAATTCATGGAAGCCTTGATACCAGTGATAA CGGCACCGATATTATACAGCTTC CAAAGCAGCGACAACGAATC GTAACCCGCTGAACCTCC | GGCCCAAAAGGAATCAACTA AAGCTTCTACCACAGATGTATTTCTCTGATCT AAGCTTCTACCACAGGTGAGTCTCGCGAACT AAGTTGAAGAACCAAGGGACACC CTCAGGCAGAAGATTGATGGG GTCCGAAGACCTCACTAAATCA |
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